TV Tuner

About

TV Tuner is an add-in for ActiveView2 (AV2) to help measure and optimize the single pulse response (SPR) of the system.

How it works

TV Tuner is centered around the concept of Procedures, which serve as organized sets of instructions for data collection and analysis. Within each Procedure are Steps, encompassing specific parameter ranges and settings to acquire and analyze. These Steps are further broken down into Results, representing distinct combinations of parameters.

The software utilizes this information to generate patterns in AV2 corresponding to each Result. When a Procedure is run, these patterns are executed sequentially, with inter-step delays to accommodate for cell pressure stabilization during gas flow changes.

Automatic data collection functionality retrieves, processes, and displays data in real-time as the Procedure progresses. Alternatively, manual data retrieval is possible, allowing users to assign data to entire Steps or individual Results as needed.

When the data for a Result become available, data processing, facilitated by iolite, occurs behind the scenes, analyzing peak locations and various statistical measures of the SPR.

Upon completion of a Procedure, suggested parameters are provided for each Step, though users can exercise their analytical discretion when choosing the best combination of parameters.

Results can be stored in a Library for future reference, and the software offers options to email or create a zip file containing Procedure details, data, and SPR measurements.

Installation

Note

TV Tuner requires AV2 version 1.5.1.33 or later (pipeline 1.0.0.5) and iolite 4.9.0 or later.

1. Obtain the TV Tuner installer from the iolite store

2. Run the installer on your AV2 computer

3. Activate the add-in using AV2's settings ( button, bottom left) → Manage Addin

4. Install and register iolite 4 on the AV2 computer (see the iolite installation guide for details)

5. Import a file from your mass spectrometer in iolite to be sure that, for example, the timestamp format is set and iolite is able to import your mass spectrometer files

TV Tuner can automatically receive data from the mass spectrometer in three ways: using the Chronicle File Watcher (CFW), a built-in file watcher, or the icpTOF web service. For more information about CFW or the icpTOF web service, see the AV2 manual or contact Tofwerk, respectively.

User interface

The TV Tuner user interface is divided into four tabs: Tune, Library, Editor, and Settings.

Tune

Fig. 33 The Tune tab

The Tune tab consists of 5 sections:

Procedure

This section is where you can select a Procedure, create AV2 laser patterns for the selected Procedure, and run the created patterns.

Stabilization

This section is where you can view the cell pressure as a function of time. This can be helpful to visually validate your selected stabilization criteria (see Settings).

Results

This section is where SPR information will be visualized as the data become available and are processed by iolite. If a Step consists of a single variable parameter, the Results will be visualized as a statistical plot, whereas if the Step consists of two variable parameters, the Results will be visualized as a heatmap (shown in Fig. 33).

Recommendations

This section considers all of the results for each Step and suggests a parameter combination deemed optimal. If targeting peak width, it is calculated as the minimum (or maximum) peak width while also maintaining greater than the median peak height.

Actions

This section allows you to send the current run to the Library, via email, or to a zip file. It also allows you to apply all recommendations via a single button.

Note

Each section can be expanded or collapsed as needed by clicking the round arrow button to the left of the section name.

Selecting and running a procedure

The active Procedure is selected via the combo box in the Procedure section. This combo box will contain the built-in procedures as well as any custom procedures you have created (see Editor).

TV Tuner comes with three built-in Procedures:

Current Single Pulse Response

Creates a single tuning ablation line that can be used to determine the current SPR for the current laser conditions.

Minimize TV3 Peak Width

A two-Step Procedure that first optimizes the Z-offset (step 1) and then optimizes the cup and cell flow rates (step 2) for minimum peak width.

Maximize TV3 Peak Height

A two-Step Procedure that first optimizes the Z-offset (step 1) and then optimizes the cup and cell flow rates (step 2) for maximum peak height.

Clicking the View button beside the combo box will take you to the Editor tab where you can view and edit the selected Procedure's details.

Note

Built-in Procedures cannot be edited. You can copy a built-in Procedure to create a custom version that can be edited.

Beneath the combo box is a description of the selected Procedure. This description can be edited in the Editor tab. The estimated run time for the Procedure is also shown. The estimate is calculated based on the laser pattern durations and the estimated cell pressure stabilization time.

Once you have selected the Procedure you want to run, you can create the patterns (tuning ablation lines) required for the Procedure by clicking the Create Patterns button. The patterns will be created at the current stage position. You should position these patterns over your tuning sample so that they avoid any surface areas that may cause signal anomalies.

Once your patterns have been created, the Steps for the Procedure are added to the Results section of the Tune tab. Expanding each Step will show the Result (ablation line) associated with each combination of tuning parameter values. Initially each Step and Result will have a status of 'Waiting'.

When you are ready to run the Procedure, click the Run Procedure button. Ablation of each line will begin after the tuning parameters have been set and the cell pressure has stabilized (Fig. 34). The stabilization plot can optionally show the slope of the cell pressure versus time for the last 15 seconds. The stabilization criteria can be set in the Settings tab.

Fig. 34 The Stabilization section of the Tune tab

If one of the automatic data collection mechanisms is enabled, the data for each tuning line will be retrieved and processed after each line is finished. The status for each result will change from 'Acquiring' to 'Processing' to 'Complete' as iolite reads in the data and determines the SPR for the data. If there is an error with processing the data, the status will change to 'Error'. If you are not using automatic data collection (see Editor), a Select Data button will be shown in the Results section of the Tune tab. Clicking this button will allow the analyst to select a file containing all the tuning data. Alternatively, you can right click on a specific Step or Result and select 'Select Data' from the context menu to assign a file to that specific part.

Once some data have been received, clicking on the Step in the Results section will plot a summary of the SPR data for that Step. If the Step contains just one parameter being adjusted, the plot will show the selected SPR metric (e.g., Width Mean) vs the parameter being adjusted (Fig. 35). If the Step contains two variable parameters, the plot will be a heatmap showing the selected SPR metric as a color versus the parameters being adjusted (Fig. 36). If you let the mouse linger over a point in the heatmap, a tooltip will show the data for that specific result.

Fig. 35 The Results plot showing a single parameter tuning Step

Fig. 36 The Results plot showing a two parameter tuning Step

For single parameter Steps (e.g., optimizing the Z-offset) you can adjust which SPR metric (peak width, height or area) is being displayed in the plot by clicking the dropdown menu to the top left (for the left axis) or the top right (for the right axis). Similarly, selecting a metric from the dropdown menu to the top right of the heatmap will change which parameter is visualized.

Double-clicking on any tuning result in the Results plot or on the name of a tuning line in the list of Results, will open a window showing more details about that tuning line (Fig. 37).

Fig. 37 Detailed information regarding the SPR analysis for one Result

In the Details window, the top-most plot shows where the peaks were found, with the location of the peak max shown as a red 'v', and the position at which the width was measured marked by red filled dots. The second from top plot shows the mean peak superimposed on top of each individual peak. The third from top plots show the peak area and width (including standard deviation shaded in red) in pulse order to show if these values are evolving within a tuning line. The bottom row shows histograms of the area and width to help describe the variation in these values. All plots can be zoomed using the mouse wheel or right-clicking + dragging, and panned by left-clicking + dragging. Additionally, the plots can be rescaled, copied or saved using a right-click context menu.

If you are running a multi-step procedure, a small dialog will popup between steps that allows you to apply the recommended parameters, another set of parameters of your choosing or to move on to the next step without making any changes.

Fig. 38 The dialog that appears between steps in a multi-step procedure

Library

Fig. 39 The Library tab

The Library tab consists of 5 sections:

Saved Runs

This section lists all the previously-run and saved Procedures. Clicking on a an entry in the table will display the associated notes, results, and recommendations.

Notes

This section allows you to add notes to a saved run. These notes will be saved for future reference.

Results

This section is identical to the Results section in the Tune tab. It shows the results of the selected Procedure.

Recommendations

This section is identical to the Recommendations section in the Tune tab. It shows the recommendations for the selected Procedure.

Actions

This section is similar to the Actions section in the Tune tab. It allows you to email or create a zip file of the results, or to apply the recommendations.

Note

You can delete one or more saved runs by selecting them in the table, right-clicking and selecting 'Delete selected runs'.

Note

You can view the details of a saved run's procedure by clicking the icon in the table.

Editor

Fig. 40 The Editor tab

The Editor tab is where Procedures are created or edited. As mentioned above, TV Tuner comes with three built-in Procedures. These can be used as templates for custom tuning Procedures, but cannot be edited or deleted. Any custom Procedures that you create can be edited or deleted.

The Editor tab consists of 4 sections:

Procedure

This section is where you select the procedure being edited. From the Actions menu, you can create new procedures, copy existing procedures, edit the name of a procedure, delete a procedure, and save a procedure. You can also import and export procedures to share with others or backup.

Starting Parameters

This section shows the parameters that will be applied to patterns by default. You can click the Use Current button to retrieve the current values from AV2.

Steps

This section is where you configure the parameters to be varied and their range for each step.

Procedure Options

This section provides a few additional options for how the laser and data collection will work for the procedure.

Procedure management is done primarily through the Actions button. This button opens a menu with actions: New, Clone, Rename, Delete, Export, Import, Save Current, and Save All.

When creating a new Procedure, you will be prompted for a name. You can then also add an optional description of the Procedure in the Description field.

New Procedures initially have no Steps. You can add, remove and reorder Steps for the Procedure using the Steps section. Click the Add button to add a Step to a Procedure. The added Step will have a default name (e.g. 'Step 1'), but this name can be edited by double-clicking the name. If you need to remove a step, the Remove button can be used. The Steps of a Procedure will be run in order. You can adjust the order of the Steps using the 'Move up' and 'Move down' buttons.

The Step's "Mode" determines how the Step is analyzed. At the moment, SinglePeakResponse is the only available mode, but other modes such as 'Steady State' will be available in upcoming versions. Clicking the button to the right of the Step's mode opens a dialog to set the number of pulses to be used for each tuning ablation line within the Step, the line spacing, the spot overlap, what percentage of the peak max to use to measure the peak width, and which channel to use (Fig. 41).

Fig. 41 The step options dialog

Note

You can modify the step options for a procedure and then right-click a procedure's step in the results tree to reprocess data that has already been collected with different parameters (e.g., to see how a different channel or % height for width looks).

The Optimize combo box selects which metric (peak width, height or area) will be optimized in the Step, and whether to target the minimum or maximum value.

The Parameter table shows the range of values, and the step size, for each tuning parameter. Clicking the button adds a parameter for tuning; clicking the button removes the selected parameter. The parameters available include: cup flow, cell flow, z-offset, fluence, spot size, and repetition rate. If a step uses 2 or more parameters, every combination of the parameters in the specified ranges will be determined.

If a Step consists of two variables, you have the option to exclude certain combinations of parameters. This can be useful if you know that certain combinations are not valid or are not of interest. To exclude a combination, click the button to the right of the parameter table. This will open a small dialog where you can click which combinations to exclude (Fig. 42).

Fig. 42 The exclusions dialog

The Procedure options include what type of automatic data collection to use, whether the laser energy is specified as fluence or energy percent, and lastly whether the aperture is XYR or IVA.

Settings

Here is the settings section

Fig. 43 The Settings tab

The Settings tab consists of 4 sections:

Mass Flow Controllers

In this section you can configure the ramp rate when changing the cell or cup flow rate as part of a tuning step.

Cell Pressure

In this section you can configure which pressure meter is used for cell pressure and the stabilization criteria. Stability is measured as the slope of the pressure versus time for the last 15 seconds.

File Watcher

In this section you can configure the path to watch and the mode.

icpTOF Web Service

In this section you can configure the URL of the web service and select which channel you want to use for SPR measurements. You can click the Get options button to connect to the icpTOF web service and determine which channels are available. You can also toggle whether you want TV Tuner to toggle TofDaq acquisition when patterns start/stop.

Other

In this section you can find buttons to open the log file and check the mass flow controllers.

General workflow

Before using TV Tuner you should have started, warmed up, and run your typical tuning procedure for the laser and mass spectrometer. TV Tuner does not tune any of the mass spectrometer settings, so it should be as accurately tuned as possible before running a Procedure. The general workflow is as follows:

1. Perform any setup required for automatic data capture. See Settings, Editor, Nu Vitesse, Tofwerk icpTOF for more information.

2. Place a tuning sample in the cell.

3. Move the stage to an area that will accommodate the tuning patterns.

4. Select the Procedure you want to run or create a new one. See Tune and Editor.

5. Create the tuning patterns. See Tune.

6. Verify the pattern locations are valid (e.g., don't extend beyond the sample).

7. Run the procedure. See Selecting and running a procedure.

8. Optionally watch the stabilization plot as the gas flow rates change and the cell pressure stabilizes (The Stabilization section of the Tune tab).

9. If using automatic data collection, browse the results as they come in (See Results).

10. If not using automatic data collection, wait for the procedure to finish, fetch the data, and select the data to process.

11. Save or share the outcome by emailing, zipping, or sending to the built-in Library. (See Actions).

Note

The Run Procedure button is replaced by a Cancel Procedure button while a procedure is running. If something does not look right while a procedure is running, you can click the Cancel Procedure button to abort.

Note

Be sure that you have calibrated the stage before running a tuning procedure. If the stage is not calibrated, the patterns will not run properly.

Other considerations

Nu Vitesse

If using a Nu Vitesse instrument, automatic data collection can be achieved by using the file watcher. In the settings, you should specify the path as a network share of the Vitesse data and the mode to Vitesse.

Note

You can export the TV Tuner pattern list for use in CoDaq by clicking the small button in the top right area of the Results section of the Tune tab.

Note

Information regarding CoDaq setup is forthcoming. In the meantime, please contact Nu Instruments for more information.

Tofwerk icpTOF

Automatic data retrieval can be accomplished with the icpTOF web service. If this was not installed with Tof Pilot, you can download it here. You can start the web service by double clicking the TofDaqWS.exe file. You can then configure the URL that TV Tuner will connect to in the Settings tab. You can also click the Get options button to determine which channels are available and toggle whether you want TV Tuner to start/stop TofDaq acquisition when patterns start/stop (recommended).

Fig. 44 The icpTOF web service

You will need to access TofDaqRec to configure the acquisition settings. Open the window from the task bar or from within TofPilot and then click the "Edit config" button.

Fig. 45 TofDaqRec window

For the DAQ settings, it is necessary to use the "STICK" firmware. We also recommend that auto saving is disabled and "none" is selected for external block trigger (Fig. 46).

Fig. 46 TofDaqRec DAQ settings

For the basic TOF timing settings, it is necessary to increase the number of segments recorded. The settings shown in Fig. 47 worked well in our testing.

Fig. 47 TofDaqRec basic TOF timing settings

If something goes wrong

Please contact support@iolite-software.com if you have any problems. When you email, please include the TV Tuner log (can be accessed through Settings tab), the procedure (can be exported, see Editor), and any data files that were collected. Depending on what you're having a problem with. It may be possible to zip a complete run from the actions and share that.

Email

If you want to use the email action, you will need to configure the email settings via AV2.

Peak measurements

The general peak finding and measurement system is outlined below.

Note

All operations are done on the channel specified in the step options. If you're using a quadrupole instrument, you may want to limit the acquisition to 1 analyte so that the cycle time can be kept short to achieve adequate time resolution. If using a TOF instrument, you may want to consider changing iolite's import settings such that you only consider useful channels (to avoid being dominated by a very high background channel) if using "TotalBeam".

1. The background mean and standard deviation are determined from the lowest parts of the data.

2. The expected peak locations are determined by creating a box train signal according to the rep rate and syncing it with the data.

3. Spurious peaks are filtered out in parts of the data where we don't expect peaks.

4. Peaks are found using SciPy's "find_peaks" moving backwards through intensity percentiles to see how many peaks are found.

5. Stop moving backwards once the expected number of peaks are found (or close to it).

6. SciPy's find_peaks uses a distance related to the repetition rate, height according to percentile, and rel_height according to the step options % height for width.

7. The information from this script is transferred back to TV Tuner for further plotting and analysis.

Support

If you have any problems, suggestions or questions, please do not hesitate to contact us at support@iolite-software.com.

Glossary

AV2

ActiveView2, the laser control software

CFW

Chronicle File Watcher, an application for automatically sending files from the mass spectrometer computer back to AV2.

Parameter

A tuning parameter, e.g. the Cell gas flow rate.

Procedure

A set of tuning Steps, executed in order to determine the best overall tuning parameters.

Result

A placeholder or outcome of SPR analysis for a specific combination of parameter settings.

SPR

Single Pulse Response: the typical peak measured by the mass spectrometer from a single laser pulse. Typically the SPR is described by its height (the max counts over the peak) and its width. This plugin also reports the average peak area. The width is usually described at some percentage of the max height, e.g. the width at 1% of the max height. Due to the noise associated with any one pulse measurement, these values are usually reported as the average of many individual pulses.

Step

A part of a Procedure where one or more tuning parameters are varied to observe the effect on the pulse signal (e.g. pulse height, pulse width etc) as measured by the mass spectrometer.